体外成牙环境对牙髓干细胞和外胚间充质干细胞分化的影响
Effects of tooth germ microenvironment in vitro on the differentiation of dental pulp stem cell and ectoblast mesenchyme stem cell
作者：王亦菁1 张晓东1 于华1 金岩2 史俊南3
Author：Wang Yijing1, Zhang Xiaodong1, Yu Hua1, Jin Yan2, Shi Junnan3
收稿日期：2012-06-16 年卷（期）页码：2012,30(6):579-579-583
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙胚细胞, 牙髓干细胞, 外胚间充质干细胞, 细胞分化,
Key words：tooth germ cell, dental pulp stem cell, ectoblast mesenchyme stem cell, cell differentiation,
基金项目：
中文摘要
目的利用牙胚细胞（TGC）作为诱导成牙环境，将牙髓干细胞（DPSC）、外胚间充质干细胞（EMSC）分别与其共培养，观察DPSC和EMSC的分化能力。方法利用出生后4 d的大鼠TGC作为诱导成牙环境，将培养的DPSC、EMSC使用BrdU标记及鉴定后与其共培养，免疫荧光双标检测标记细胞表面抗原牙本质涎蛋白（DSP）的表达和碱性磷酸酶（ALP）活性的变化，免疫组化染色鉴定及图像分析DPSC、EMSC在成牙环境中的分化能力。结果共培养7 d后，EMSC共培养组DSP阳性细胞的转化率高于DPSC共培养组（P
英文摘要
Objective To observe the differential ability of dental pulp stem cell（DPSC） and ectoblast mesenchyme stem cell（EMSC） that were cultured with tooth germ cell（TGC） as the tooth germ microenvironment. Methods The TGC of 4-day old rat was used as the tooth germ microenvironment. The BrdU marked and determined DPSC and EMSC were cultured with the TGC respectively. The expression of cell surface antigen dentin sialoprotein（DSP） and alkaline phosphatase（ALP） activity were determined with double marker immunofluorescence. The differential ability of DPSC and EMSC were determined by the immunohistochemistry and image analysis in the tooth germ microenvironment. Results The transformation efficiency of DSP positive cell in the EMSC co-culture group was higher than that in the DPSC co-culture group（P
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