骨髓间充质干细胞对机械张应力刺激的反应及转化生长因子-β和胰岛素样生长因子-Ⅱ的基因表达

Objective To study the response of rat bone marrow mesenchymal stem cells（MSCs） to a single period of mechanical strain and expression patterns of transforming growth factor-β（TGF-β） and insulin-like growth factor- Ⅱ（IGF-Ⅱ） after mechanical stretch． Methods Bone marrow MSCs were isolated from SD rats and cultured in vitro. A four-point bending apparatus were used to perform a single period of mechanical strain（2 000 με, 40 min） on MSCs. Cellular proliferation and alkaline phosphatase（ALP） activity of MSCs were examined and gene expression patterns of TGF-β and IGF-Ⅱ were detected by SYBR green quantitative real-time RT-PCR． Results Cell proliferation, ALP activity and expression of TGF-β and IGF-Ⅱ were all significantly up-regulated in stretched MSCs when compared with their controls. The mRNA levels of TGF-β and IGF- Ⅱ got top increase immediately after mechanical loading and increased about 51.44 and 8.92 folds, respectively, when compared with control cells. Expression of TGF-β and IGF-Ⅱ decreased with time and returned to control level at 12 h after mechanical stimulus, despite of a small increase at 6 h. Conclusion The mechanical stretch can promote MSCs proliferation, up-regulate its ALP activity and induce a time-dependent expression increase of TGF-β and IGF-Ⅱ which in turn result in osteogenic differentiation of MSCs. Mechanical stimulus is a key stimulator for osteogenic differentiation of MSCs and vital for bone formation in distraction osteogenesis.