Objective To investigate the frequency and intensity of Candida spp. incidence from the oral cavities of the healthy elderly in Chengdu, and to study the role of the dentures in the distribution of oral Candida spp. Methods A total of 212 individuals（age >60 years） were divided into four groups: A1（48 man with dentures）, B1（61 man without dentures）, A2（53 women with dentures） and B2（50 women without dentures）. Samples of their oral flora were obtained by rinsing with 10 mL PBS solution. The samples were centrifuged and resuspended in PBS（500 μL）, and plated onto Sabouraud's dextrose agar. CHROMagar CandidaTM, sugar assimilation patterns（API 20C AUX tests） were used to determine Candida spp. The total number of yeast colonies on the plates was considered as the relative intensity of oral Candida. Results Candida spp. was isolated from 116 healthy elderly individuals（54.72%）, such as C.albicans, C.parapsilosis, C. krusei, C.guilliermondii, C.tropicalis, etc. The frequency of Candida spp. in A1, B1, A2 and B2 was 66.67%, 36.07%, 64.15%, and 56.00%, respectively. The frequency of C. albicans in A1, B1, A2 and B2 was 56.25%, 21.31%, 56.60% and 38.00%, respectively. The frequency of Candida spp. and the intensity of Candida spp. were greater for individuals in the denture-wearing group than that in the control. Conclusion The frequency and intensity of Candida spp. incidence from the healthy elderly are closely correlated with denture-wearing, and the differences of the frequency and intensity of Candida spp. incidence in the elderly are due to the differences of frequency and intensity of C.albicans incidence.
