三氧化二砷对舌鳞癌细胞凋亡及端粒酶逆转录酶基因的作用
Effects of Arsenic Trioxideon the Cell Apoptosis and hTERT mRNA of Human Tongue Cancer Cells
作者：姚华,吴求亮,王慧明,范骏
Author：YAO Hua1,WU Qiu-liang1,WANGHui-ming1,FANJun2
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):442-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：三氧化二砷,舌鳞癌细胞,细胞凋亡,端粒酶逆转录酶,
Key words：arsenic trioxide,human tongue cancer cell lines,cell apoptosis,human telomerase reverse transcriptase,
基金项目：浙江省卫生厅资助项目(2003B058)
中文摘要
目的 研究三氧化二砷(As2O3)对舌鳞癌细胞株(Tca8113)增殖的影响及对端粒酶逆转录酶基因(hTERT
mRNA)的作用,并初步探讨三氧化二砷的作用机制。方法 以舌鳞癌细胞株(Tca8113)为研究对象,采用噻唑蓝
(MTT)、Annexin V/碘化丙锭(PI)染色法检测细胞生长抑制率和细胞凋亡率;采用Western blot检测细胞hTERT蛋白
的表达;采用RT-PCR法检测细胞hTERTmRNA的表达情况。结果 As2O3能够明显抑制Tca8113细胞增殖,诱导
细胞凋亡,并有浓度、时间依赖性。5μmol/L As2O3组72 h细胞生长抑制率为83·40%±7·31%,细胞凋亡率为
26·40%±3·42%。As2O3能抑制hTERTmRNA的表达和翻译,随As2O3作用时间、浓度增加,细胞hTERTmRNA和蛋
白表达减弱,各实验组与对照组比较差异有统计学意义(P
英文摘要
Objective To study the effects and its mechanisms of arsenic trioxide (As2O3) on cell growth and human telomer- ase reverse transcriptase (hTERT) of human tongue cancer cells (Tca8113 cell line).Methods The growth inhibition rates of Tca8113 by various concentrations of As2O3were detected byMTTmethod. Cell apoptosis was detected by FCM labeled with An- nexin V-FITC. hTERT gene expression was detected by RT-PCR method. hTERT protein of Tca8113 cells was determined by Western blot assay.Results The results showed that As2O3could inhibit the growth of Tca8113 effectively and apoptotic rate of Tca8113 cells was obviously increased in a dose and time-dependent manner. (83·40±7·31)% cells treated with 5μmol/L As2O3were inhibited on 72 hour point, the early apoptosis rate reached on 26·40%±3·42% at that time. Moreover hTERTmR- NA and proteinwere decreased depended on the dose and time of As2O3, mRNA expressions of hTERT in test groupswere greatly lower than that of control group on 72 hour point.Conclusion It was suggested that As2O3could significantly inhibit the growth of Tca8113 cells by inducing causing cell apoptosis and down-regulating the expression of hTERT mRNA gene and protein which might be one of its action mechanisms.
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