口腔癌相关成纤维细胞的染色体核型分析
Analysis of Chromosome Karyotype of Oral Carcinoma-associated Fibroblasts
作者：郑晓辉,刘英,周红梅,陈谦明,李秉琦
Author：ZHENGXiao-hui1,LIUYing2,ZHOUHong- mei2,CHENQian-ming2,LI Bing-qi2
收稿日期：2005-04-25 年卷（期）页码：2005,23(02):159-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：成纤维细胞,鳞状细胞癌,染色体,核型,
Key words：fibroblast,squamous cell carcinoma,chromosome,karyotype,
基金项目：高等学校博士学科点专项科研基金资助项目(20030610051);教育部回国人员启动基金资助项目(200340606)
中文摘要
目的 通过对口腔癌相关成纤维细胞和口腔黏膜正常成纤维细胞染色体核型的对比分析,初步了解口腔癌相关成纤维细胞的遗传学基本特征是否发生变化。方法 用秋水仙素、低渗液处理两种细胞,经冰醋酸膨胀及甲醇固定后,进行Giemsa染色,在油镜下观察细胞染色体,并采用Visus 2.1软件对细胞染色体核型进行分析。结果 与口腔黏膜正常成纤维细胞比较,口腔癌相关成纤维细胞的染色体核型未见明显差异。结论 口腔癌相关成纤维细胞仍保留正常细胞的基本遗传特征,未发生恶性转化。
英文摘要
Objective The purpose of this study was to investigate whether the fundamental genetic character of oral carcino- ma-associated fibroblasts changes through contrasting and analyzing the oral carcinoma-associated fibroblasts and the normal fibrob- lasts of oral mucosa.Methods The two kinds of cellswere treatedwith colchicine and microsometic fluid, and thenwere expand- ed with cold acetic acid and formalized with methyl alcohol. The cells were observed under the oil microscope after Giemsa stain- ing. The chromosome karyotype of the two kinds of cellswas analyzed by Visus 2.1.Results There were not obvious differences in the way of chromosome karyotype between the oral carcinoma-associated fibroblasts and the normal fibroblasts of oral mucosa. Conclusion The basic genetic characteristics of the normal cells are conserved in the oral carcinoma-associated fibroblasts, which means the cells have no malignant changes.
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