Objective The aims of the present studywere to assess the characteristics ofMSCswhichwere induced and prolif- eratedin vitro, then to learn the interfaces between them and metal materials.Methods Adult dogswere selected as experimen- tal animals, the MSCs (marrow stem cells) were aspirated from the femur, which were induced to the phenotype of OB (osteo- blast), then to proliferatedin vitrowith metal materials. Using biochemical tests, phase contrast microscopy, transmit electromi- croscope, scanning electromicroscope to detect MSCs and the image of interface.Results MSCs had powerful osteogenic pheno- type when they were inducedin vitro, and they also attached to the metal materials. And there were more cellswhich attached on tensile stress area than other areas.Conclusion The MSCs inducedin vitrocould be used as source of osteoblast. The affects of air-abrasion with alumina are not significant, but they have relation to the extent of strain and property of stress. [Key words] marrow stem cells|cell culture|osteogenisis|interface|strain
