成骨细胞和牙周膜成纤维细胞交互作用的初探
Interaction between Osteoblasts and Fibroblasts Derived from Same Individual
作者：宫苹,谭震,赵青
Author：GONG Ping,TAN Zhen,ZHAO Qing.
收稿日期：2004-08-25 年卷（期）页码：2004,22(04):275-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙周膜成纤维细胞,成骨细胞,细胞共同培养,
Key words：periodontal ligament fibroblast,osteoblast,cell co-culture,
基金项目：
高等学校博士学科点专项科研基金资助项目 (B120010610059)
中文摘要
目的 了解牙周组织再生中两种重要的细胞(成骨细胞和牙周膜成纤维细胞)之间的相互调控作用,以探讨牙周膜形成和维持的相关因素及其对牙种植体周围结构的影响机制。方法 采用原代培养法获取同一SD大鼠的成骨细胞和牙周膜成纤维细胞,将两种细胞分别接种于培养板上,再置于细胞培养池中以建立体外共同培养模型,分别测试两种细胞的碱性磷酸酶(ALP)及Ⅰ型胶原表达。结果 两种细胞在共同培养后,其ALP活性都发生显著变化,牙周膜成纤维细胞ALP表达明显提高,而成骨细胞ALP则降低;Ⅰ型胶原未发生显著变化。结论 当两种细胞在体外共同培养时,牙周膜成纤维细胞能抑制成骨细胞的成骨作用,而成骨细胞则能诱导牙周膜成纤维细胞向成骨样细胞分化。
英文摘要
Objective In order to determine the effect of factors pertaining to the development and metabolism of periodontal ligament, the author investigated the interaction between osteoblasts and fibroblasts.Methods The cell co-culture model was es- tablished by cell culture inserts. The expression difference of protein related to mineralization (ALP, TypeⅠcollagen ) between these two kinds of cells cultured individually and co-cultured were evaluated.Results Co-culture changed the ALPase activity of the two kinds of cells greatly .The ALP expression of osteoblasts decreased, but the ALP expression of periodontal ligament fibro- blasts incresed. The Col-Ⅰexpression of these two kinds of cells did not change significantlywhen theywere co-cultured.Conclu- sion Periodontal ligament fibroblast could restrain the osteogenesis function of osteoblast, while osteoblast could induce the dif- ferentiation of fibroblast when both of them existed in periodontal tissue.
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