唾液腺粘液表皮样癌组织P-gp单克隆抗体JSB-1及GST-π抗体的检测
Detection of P-glycoprotein and Glutathinoe S-transferase in Mucoepidermoid Carcinoma of Salivary Gland
作者：何 嘉1,王大章2,郑光勇1,冯 戈1
Author：HE Jia1, WANG Da-zhang2,ZHENG Guang-yong1,FENG Ge1
收稿日期：2004-04-25 年卷（期）页码：2004,22(02):115-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：多药耐药,P糖蛋白,谷胱苷肽-S转移酶,粘液表皮样癌,
Key words：multidrug resistance,P-glycoprotein,glutathione,mucoepidermoid carcinoma,
基金项目：
国家自然科学基金资助项目(0112444);四川大学优秀博士论文奖励基金资助项目(40305505010)
中文摘要
目的 探讨唾液腺粘液表皮样癌耐药性产生的机制,以便有针对性地采取逆转措施提高综合治疗中化疗的效果。方法 选取四川大学华西口腔医院口腔颌面外科1995~2000年40例术前未经任何治疗的唾液腺粘液表皮样癌标本,采用免疫组化ABC法,进行P-gp单克隆抗体JSB-1的检测,同时对其中10例进行了GST-π的检测。结果 JSB-1表达阳性率为67·5%(27/40),与分化程度相关,高分化组和中分化组均高于低分化组(P0·05)。GST-π表达阳性率为90%(9/10)。两种抗体的表达在唾液腺粘液表皮样癌组织中的表达无统计学差异,亦无相关性。结论 JSB-1及GST-π与唾液腺粘液表皮样癌产生耐药的机制有关,为开展其耐药性的研究提供了靶标。
英文摘要
Objective The aim of this studywas to investigate the mechanism(MDR) ofmultidrug resistance(MDR) ofmucoe- pidermoid carcinoma in salivary gland.Methods 40 cases of mucoepidermoid carcinoma in salivary gland were examined the MDR gene product P-glycoprotein using a monoclonal antibody JSB-1. And 10 of themwere also investigated by detecting the ex- pression of GST-π. All the cases had not been accepted any therapy before the samples were collected.Results ①Positive ex- pression of JSB-1 was observed in 27 of the 40 specimens. The positive expressionwas related not onlywith clinical stage, but also with differentiation degree.②The GST-πpositive expressionwas found in 9 of 10 cases. Therewas no significant different between the positive expression of JSB-1 and GST-π.Conclusion JSB-1 and GST-πplay an important role in MDR of mucoepidermoid carcinoma.
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