人血管内皮生长因子-C基因编码区cDNA的克隆与测序
Cloning and Sequencing of Human Vascular Endothelial Growth Factor-C Encoded cDNA
作者：潘剑,华成舸,温玉明,李珉,王刚,王昌美,李龙江,陈绍维
Author：PANJian*,HUAChengge,WEN Yuming,et al
收稿日期：2003-08-25 年卷（期）页码：2003,21(04):318-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：血管内皮生长因子-C,分子克隆,DNA序列测定,
Key words：vascular endothelial growth factor (VEGF)-C,molecular cloning,DNA sequencing,
基金项目：本课题为国家自然科学基金资助项目(编号39970796)
中文摘要
目的 克隆人舌癌组织中血管内皮生长因子(VEGF)-C功能片段的cDNA,为进一步研究VEGF-C在口腔鳞癌中表达的意义及与淋巴转移的关系打下基础。方法 以RT-PCR法从舌鳞癌组织中克隆VEGF-C基因功能片段的cDNA,用限制性内切酶消化和PCR筛选出阳性克隆pCRII-VEGF-C,以SP6和T7引物完成VEGF-C cDNA 的全序列测定。结果 从人舌癌组织总RNA中克隆到约1·1 kb大小的VEGF-C基因cDNA,经测序与Genbank公布的人VEGF-C基因cDNA序列的同源性为99·6%。结论 从人舌癌组织中克隆得到的VEGF-C基因功能片段cDNA, 为进一步研究VEGF-C在口腔癌颈淋巴结转移中的功能效应提供了物质基础。
英文摘要
Objective A functional VEGF-C cDNA was cloned from a patient with squamous cell carcinoma (SCC) of tongue in orderto study the important role of vascular endothelial growth factor (VEGF)-C in lymphatic dissemination of malignan- cies in gene level.Methods RT-PCR was employed to clone the human VEGF-C encoded cDNA from a surgical specimen of a lingual SCC patient. Then itwas subcloned into plasmid vector pCRII and sequenced.Results A1·1 kb human VEGF-C cDNA fragmentwas amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99·6% similarity with the reported human VEGF-C cDNA (representing the 559~1 611 bp according the sequence of Genbank Entry X94216). Conclusion An encoded fragment VEGF-C cDNAwas successfully cloned from a lingual SCC and provided a necessary material for further study.
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