变形链球菌表面蛋白和葡糖基转移酶基因疫苗免疫防龋实验研究:唾液和血清抗体水平测定
An Experimental Study on PAc and GTF Gene Vaccines ofStreptococcus Mutansagainst Rats Caries: Antibody Levels in Saliva and Serum
作者：杨德琴,刘天佳,曹福娴,杨锦波,刘建国
Author：YANG Deqin*,LIUTianjia,CAO Fuxian,et al.
收稿日期：2003-10-25 年卷（期）页码：2003,21(05):396-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：变形链球菌,表面蛋白,葡糖基转移酶,基因疫苗,
Key words：Streptococcus mutans,glucosyltransferases,surface protein antigen,gene vaccine,
基金项目：本课题为国家自然科学基金资助项目(编号39770797)
中文摘要
目的 将基因疫苗pcDNA3-pac和pcDNA3-gtfB经腺周注射免疫定菌大鼠,观察唾液和血清抗体的变化,证实基因疫苗的免疫原性。方法 36只Wistar大鼠分为6个免疫组:pcDNA3-pac,pcDNA3-gtfB,pcDNA3-pac联合 pcDNA3-gtfB,阳性对照灭活变形链球菌,阴性对照pcDNA3及PBS液组。建立龋攻击定菌鼠模型3个月,各组大鼠均以100μg剂量免疫定菌鼠3次,ELISA法检测唾液SIgA和血清IgG水平。结果 疫苗组在首次免疫后第33天 (第5周)都出现较高水平的唾液SIgA,在第75天(第11周)后达最高水平,并持续至实验结束。成组单因素方差分析唾液SIgA水平在总体上各实验组间存在差异(P0·05),但都高于空白及阴性对照组(P
英文摘要
Objective The purpose of this study is to examine the levels of salivary SIgA and serum IgG induced by pcDNA3-pac and pcDNA3-gtfB immunization, so as to testify the antigenity of the two gene vaccines.Methods 36 28-day-old Wistar rats were divided into 6 groups, among which 3 experimental groups were vaccinated with pcDNA3-pac, pcDNA3-gtfB or pcDNA3-pac combined with pcDNA3-gtfB, respectively, one positive control was vaccinated with inactive whole cell ofS.mutans JBP and other two negative controls were injected with the vector pcDNA3 or PBS buffer, respectively. All vaccines and materials were delivered with 100μg by submandibular gland injection for 3 times. Then the restricted bacterial model of ratwas construct- ed. Following that all ratswere fedwith cariogenic dietKeyes 2000#for 3 months, saliva and serum sampleswere collected to as- say SIgA or IgG levels by ELASA.Results The salivary S-IgA levels both in pcDNA3-pac combined with pcDNA3-gtfB group and inactiveS.mutanscell group were higher than others (P
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