牙本质涎磷蛋白反义核酸对牙胚超微结构的影响
Effects of Dentin Sialophosphoprotein Antisense Oligodeoxynucleotide on Ultrastructure of Mouse Tooth Germ
作者：张蓉,肖明振,赵守亮,徐平西,张春宝,汪平
Author：ZHANG Rong*,XIAOMingzhen,ZHAO Shouliang,et al.
收稿日期：2003-08-25 年卷（期）页码：2003,21(04):281-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙本质涎磷蛋白,反义核酸,牙胚,超微结构,
Key words：dentin sialophosphoprotein(DSPP),antisense oligodeoxynucleotide,tooth germ,ultrastructure,
基金项目：
本课题为国家自然科学基金(编号39800155)及全军“九五”重点课题(编号98Z082)资助项目
中文摘要
目的 探讨牙本质涎磷蛋白(DSPP)在牙胚发育和矿化中的作用及其机制。方法 胎龄为17 d Balb/C 胎鼠上颌第一磨牙,分为2组,对照组牙胚置于无血清BGJb半固定培养基中培养;DSPP反义核酸组牙胚置于含有 30μmol/L,长15 bp的DSPP反义寡核苷酸的半固体培养基中培养。牙胚体外培养10 d后,进行透射电镜观察。结果 2组牙胚牙尖处成牙本质细胞中均含有大量的细胞器,线粒体肿胀,粗面内质网扩张,且DSPP反义核酸组成牙本质细胞中粗面内质网扩张更为明显;胞外基质超微结构显示:对照组胶原纤维聚集成束,排列具有一定的方向, 牙尖基质带平均厚度为3μm ;DSPP反义核酸组胶原纤维粗细不等,排列紊乱,牙尖基质带平均厚度为2·5μm。结论 DSPP可能通过控制成牙本质细胞正常的分泌功能、胶原纤维的正常形态和排列在牙胚生长、矿化中起重要作用。
英文摘要
Objective To investigate more deeply the function and mechanism of DSPP during tooth development.Meth- ods Explants of tooth germs from embryonic 17 th day mice were divided into two groups. In the control experiment, explants were cultured in agarose semi-solid medium under serum-free and chemically defined conditions, while explants in the other group were cultured with 30μmol/L, 15 bp antisense oligodeoxynucleotide targeted to DSPP mRNA. After 10 ds, the explants were ex- amined by transmission electron microscope. The width of dentin matrix at the tip of the cuspswere then measured and statistically analyzedwith Studentt-test.Results Ultrastructure analyses showed that large cisternae of the rough endoplasmic reticulum (RER) existed in the odontoblasts at the tip of the cusps of antisense-treated explants and the average thickness of dentin matrix (2.5μm) was thinner compared to the control ones (3μm,P
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