静张应力对大鼠髁突软骨细胞增殖效应调节研究
Static Tension-stress Effects on Proliferation of Mandibular Condylar Chondrocytes in vitro
作者：宋锦璘,罗颂椒,樊瑜波,赵志河,郭欣
Author：SONG Jinlin*,LUO Songjiao,FAN Yubo,et al.
收稿日期：2003-02-25 年卷（期）页码：2003,21(01):57-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：静张应力,髁突软骨细胞,增殖活性,功能矫治,
Key words：static tension-stress,mandibular condylar chondrocytes,proliferation,functional orthopedics,
基金项目：
本课题为国家自然科学基金资助项目(编号39870231,39900166)
中文摘要
目的 探讨静张应力环境对髁突软骨细胞增殖效应的影响。方法 将第4代大鼠下颌髁突软骨细胞在细胞膜式张应力施加装置上培养,检测不同血清浓度及持续不同的静张应力(5 kPa、10 kPa)在0~12 h内对髁突软骨细胞增殖活性的影响。结果 低血清培养基中,随培养时间的延长,硅胶膜上髁突软骨细胞的增殖活性受到抑制;短期(2h)的静张应力(5 kPa、10 kPa)对髁突软骨细胞细胞周期的调控影响不大;0~10 h内5 kPa静张应力组软骨细胞增殖指数随着张应力作用时间的延长不断上升,增殖指数峰值位于10 h处;0~8 h内10 kPa静张应力组软骨细胞增殖指数随着张应力作用时间的延长不断上升,增殖指数峰值位于8 h处;5 kPa静张应力组较10 kPa静张应力组对髁突软骨细胞具有更大的促增殖作用。结论 静张应力可以调节髁突软骨细胞的增殖活性。
英文摘要
Objective The purpose of this studywas to investigate the effects of static tension-stress on proliferation of man- dibular condylar chondrocytes in rats.Methods The fourth-passage chondrocytes were harvested from the mandibular condyles of 2- week-old SD rats. A continuous static tension-stresswas applied on the cells in vitro using a cellular static tension-stress device, and the proliferation of mandibular condylar chondrocytes were examined using a flow cytometry. The other 30 specimens in the control group were divided into six groups to examine the effects of calf serum on cellular static tension-stresswithout any stress. The experi- mental group consisted of 70 specimenswhichwere divided into seven groups under continuous static tension-stress (5kPa, 10kPa) for 0 to 12 hours. Multivariable analyseswere conducted to test the associations between proliferation of condylar chondrocytes and differ- ent continuous static tension stresses.Results The results showed thatthe lower calf serum inhibited the proliferation of ratmandibu- lar condylar chondrocytes. There was little effect on the proliferation of chondrocytes under continuous static tension-stress (5kPa, 10kPa) for 2 hours. The proliferation of mandibular condylar chondrocytes was promoted as the application time of stress was pro- longed (0~10 hours in 5kPa groups and 0~8 hours in 10kPa groups). The maximal proliferation appeared, when the condylar chon- drocyteswere cultured under 5kPa continuous static tension-stress for 10 hours, and 8 hours under 10kPa stress (P
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