Objective:The purpose of this study was to investigate the inhibitory effect of interleukin-10 (IL-10) on the periodontal inflammatory reaction.Methods:Cell culture and ELISAwere used to evaluate the concentration of interleukin-1B(IL-1B) in the supernatant, and MTT was used to examine the proliferation response of human gingival fibroblasts to lipopolysaccharides and IL-10.Results:IL-1Bsecreted by human gingival fibroblastswas induced by lipopolysaccharides in a dose-dependentmanner and reached the highest level in 6 h. IL-1Bwas inhibited by IL-10, and the optimal inhibitory concentration of latter was 10 ng/ml. The inhibitory effect of IL-10 in the optimal concentration to IL-1Breached the maximum in 24 h. Further the proliferation of hu- man gingival fibroblasts was also induced by lipopolysaccharides, but not affected by IL-10.Conclusion:IL-10 is a cytokine that can inhibit periodontal inflammatory reaction.
