以L929细胞为饲细胞的口腔粘膜上皮细胞体外培养
A Study of Oral Epithelial Cells Cultured with L929 Cells as Seed Cells in vitro
作者：翟弘峰,刘红,解邦杰,李森恺
Author：Zhai Hongfeng,Liu Hong, Xie Bangjie,Li Senkai
收稿日期：2002-10-25 年卷（期）页码：2002,20(05):336-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：口腔上皮细胞,饲细胞,细胞培养,
Key words：oral epithelium,seed cell,cell culture,in vitro,
基金项目：本课题为国家自然科学基金资助项目(编号30070779)
中文摘要
目的:探索口腔粘膜上皮细胞体外培养的技术和方法,为进一步采用组织工程技术构建口腔粘膜组织奠定基础。方法:取刚离乳的新西兰幼兔口腔粘膜组织小块,酶消化成单细胞悬液,接种后以L929细胞为饲细胞培养, 定期换液、传代。以相差显微镜每天动态观察细胞形态变化及生长增殖情况,细胞进行常规HE染色、免疫组化染色及流式细胞仪检查,并以扫描电镜、透射电镜观察其超微结构。结果:整个上皮细胞生长期内无成纤维细胞混杂生长,均为单一的上皮细胞,并证实为二倍体细胞。细胞可传11~13代,成活50~60 d。结论:新西兰幼兔口腔粘膜上皮细胞可在体外进行培养,在一定时间内保持增殖能力。这不仅为组织工程化口腔粘膜构建奠定了基础,而且为口腔粘膜的体外研究提供了实验模型。
英文摘要
Objective:The purpose of study aimed atinvestigating the technique of culturing oral epithelia in vitro and to setup an exper- imental model for further reconstructing oral mucosa in vitro.Methods:The oral mucosa was taken from young NewZealand rab- bits, and the mucosa was digested with enzyme and suspended in liquid to form cellular suspension. Being seeded, the cellswere cultured motionlessly. The mediumwas changed regularly and the cellswere subcultured.Results:The cultured cellswere all ep- ithelial cells without fibroblasts, and they were proved to be diploid cells. The cellswere subcultured in 1~13 generationswhich survived for50~60 days.Conclusion:The oral epithelium of youngNewZealand rabbit can be cultured in vitro, maintaining the ability to proliferate in a certain period. It is a pilot study to reconstruct oral tissue in vitro.
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