Objective This study aims to evaluate the bond strength of a self-adhesive resin cement to dentin by ethylenediamine tetraacetic acid (EDTA) and NaClO.Methods Twenty-seven freshly extracted non-carious human premolars were prepared to expose the buccal dentin and randomly divided into three groups: control group (A group), EDTA group (B group) and NaClO group (C group). All teeth were bonded to dentin using a self-adhesive resin cement after the teeth in the A group were processed with distilled water. The B and C group were processed with 3%EDTA and 1%NaClO, respectively. After 24 hours at 37 ℃ water, the shear bond strengths of the twenty-four specimens were measured. All statistical analysis was performed using SPSS 17.0 software package. Each fractured specimen was examined under dental microscope. Three new specimens were cut, and the morphologies of the cement-dentin interface were observed under scanning electron microscope (SEM).Results The shear bond strength in the A group, B group and C group was (8.55±0.63), (8.47±0.56) and (12.97±0.59) MPa, respectively. The difference between A group and B group was no statistically significant (P>0.05), whereas the difference between C group and B group (or A group) was statistically significant (PConclusion 1% NaClO can increase the bond strength of self-adhesive resin cement to dentin, but 3%EDTA has no effect.