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论文摘要

丹酚酸B对人牙周膜细胞成骨分化的影响

Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells

作者:马玥 任嫒姝 付钢

Author:Ma Yue, Ren Aishu, Fu Gang.

收稿日期:2016-01-06          年卷(期)页码:2016,34(5):468-468-473

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:丹酚酸B,人牙周膜细胞,成骨分化,碱性磷酸酶活性,矿化结节,

Key words:salvianolic acid B,human periodontal ligament cells,osteogenic differentiation,alkaline phosphatase activity,mineralized nodules,

基金项目:

重庆市渝中区科委基金(20140128);重庆市卫生局基金资助项目(2011-2-180);2013年重庆高校创新团队建设计划资助项目(KJTD201314)

中文摘要

目的 探讨中药丹参主要活性成分丹酚酸B(SalB)对人牙周膜细胞(hPDLCs)成骨分化的影响。方法 取第3代hPDLCs进行实验,运用甲基噻唑基四唑(MTT)法检测不同浓度丹酚酸B对hPDLCs增殖活性影响,通过检测碱性磷酸酶(ALP)活性、矿化结节染色、骨钙素(OCN)mRNA表达来探讨丹酚酸B对hPDLCs成骨分化的影响。结果 丹酚酸B对hPDLCs增殖活性无影响。当丹酚酸B浓度为0.5、1、5 μmol·L-1时均能增加hPDLCs的ALP活性和OCN mRNA表达,与OIM组比较差异有统计学意义(P-1时hPDLCs形成矿化结节数量明显高于OIM组。结论 适宜浓度的丹酚酸B能有效促进hPDLCs成骨分化。

英文摘要

Objective This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).Methods Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.Results Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P−1) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L−1) than that in the OIM group.Conclusion Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.

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