Objective This study investigated the rapid response of osteoblasts, which were derived from low-magnitude high-frequency vibration (LMHFV). Refractory period-derived memory response was also observed. Methods MC3T3-E1 cells were incubated and received LMHFV stimulation (0.49 g, 40 Hz) for 30 min. After application of LMHFV, mRNA levels of earlier osteogenic differentiation markers Runt-related transcription factor 2 (Runx2), collagen typeⅠ(Col-Ⅰ), and alkaline phosphatase (ALP) were immediately detected by real-time fluorescence quantitative polymerase chain reaction in the absence or presence of antioxidant. Simultaneously, concentrations of mitochondrial reactive oxygen species (ROS) and average mito-chondrial length were also measured. Results Osteoblasts in the vibration group showed decreased gene expressions of Runx2, Col-Ⅰ, and ALP (P<0 .01) and increased levels of mitochondrial ros (P<0 .01) and shortened mitochondria (P<0 .01), whereas antioxidant treatment resulted in recovery from changes in the above indicators (P<0 .01). conclusion lmhfv can downregulate mrna levels of early osteogenic differentiation markers, promote ros generation, and mitochondrial fission.
