钙离子对人成骨细胞迁移与成骨分化的影响

ObjectiveThis study aimed to investigate the effect of calcium ion (Ca²⁺) on the migration and osteogenic differentiation of human osteoblasts and explore the proper concentration and correlation mechanism.MethodsA series of Ca²⁺solutions with different concentrations was prepared. Osteoblast migration was assessed by Transwell assay, and proli-feration was studied via the CCK-8 colorimetric assay. The mRNA expression of osteogenic genes was examined via reverse transcription-polymerase chain reaction (RT-PCR), and the mineralized nodule was examined by alizarin red-S method. After calcium sensitive receptor (CaSR) antagonism, Ca²⁺-induced migration and osteogenic differentiation were analyzed.ResultsIn the migration experiment, 2, 4, and 6 mmol·L^-1Ca²⁺could promoted osteoblast migration at three timepoints (8, 16, and 24 h), whereas 10 mmol·L^-1Ca²⁺considerably inhibited migration at 8 h. The Ca²⁺concentration range of 2-10 mmol·L^-1could promote proliferation, osteogenic differentiation, and mineralization of human osteoblasts. Moreover, mineralization was predominantly induced by 8 and 10 mmol·L^-1Ca²⁺. CaSR antagonism could reduce Ca²⁺-induced migration and osteogenic differentiation of human osteoblasts.ConclusionLow Ca²⁺concentration favored osteoblast migration, whereas high Ca²⁺concentration favored osteogenic differentiation. The Ca²⁺concentrations of 4 and 6 mmol·L^-1could substantially induce osteo-blast migration and osteogenic differentiation, and the Ca²⁺-CaSR pathway participated in signal transduction.