ObjectiveTo evaluate the process characterization of graphene oxide loaded on pure titanium surface and effect on the biological properties ofStaphylococcus aureusand osteoblasts.MethodsGraphene oxide at four concentrations (20, 50, 80, and 100 μg·mL-1) was loaded on the pure titanium surface via electroplating, and the morphology, properties, and hydrophilic properties were measured with a field emission scanning electron microscope, micro Raman spectrometer, and contact angle tester, respectively. In addition,Staphylococcus aureusand osteoblasts were used as models and cultured with pure titanium-graphene oxide. Then, field-emission scanning electron microscopy and laser confocal microscopy were utilized to observe the changes in the amount of bacteria and osteoblast morphology and structure, respectively.ResultsGraphene oxide at the four concentrations was successfully loaded on pure titanium surface via electroplating. It improved the hydrophilic properties of pure titanium surface, which benefitted the adhesion and growth ofStaphylococcus aureusand changed the morphology and structure of the osteoblasts.ConclusionThe pure titanium-graphene oxide composite has no antibacterial properties and has good biocompatibility.