ObjectiveThis study aims to investigate the effect of calbindin 1 on the proliferation and apoptosis of gingival epithelial cells affected byPorphyromonas gingivalis (P. gingivalis)invitro.
MethodsA model ofP. gingivalisinfecting CA9-22 was establishedin vitro. At 24 h after infection, the expression of calbindin 1 (CALB1) was detected by real-time fluorescent quantitative polymerase chain reaction, Western blot, and immunofluorescence analyses. The expression of CALB1 was further inhibited by RNA interference. Cell proliferation was detected by BrdU analysis, and cell apoptosis was detected by caspase 3 activity. The expression of MDM2 and p53 was detected by Western blot analysis.
ResultsP. gingivalisinfection upregulated the expression of CALB1 in CA9-22 cells with multiplicity-dependent manner. CALB1 promoted the proliferation of CA9-22 cells, increased the expression of MDM2, and inhibited the expression of p53. Inhibiting CALB1 expression did not affect the inhibitory effect ofP. gingivalisinfection on CA9-22 apoptosis.
ConclusionP. gingivalisinfection can promote the proliferation of CA9-22 cells by increasing CALB1 expression. The related mechanism may be associated with MDM2-p53.
