Objective To investigate the effects of pomegranate leaves extract(PLE)on proliferation, apoptosis and metastasis of prostate cancer cells. Methods The proliferation of TRAMP-C1, DU145, PC3 prostate cancer cells treated with different concentrations of PLE (final mass concentrations were 12.5, 25, 50, 100,200 μg/mL, respectively) for different time (24, 48, 72 h) was detected by MTT assay. Colony formation assay was performed to verify the long-term effects of PLE on the proliferation of DU145 and PC3 cells.After being treated with PLE for 48 h, Hoechst-33258 staining was used to observe the changes in the nucleus, the cell apoptotic rate was detected by flow cytometry, and wound-healing migration assay was perform to test the change of migration. Results In comparison with the control group, PLE in the range of 12.5-200 μg/mL had a certain inhibitory effect on the proliferation of TRAMP-C1, DU145 and PC3 cells (PPPP
