Abstract:ObjectiveTo explore the regulatory function of long intergenic non-coding RNALINC RORthat contained within hepatocarcinoma cells-derived exosomes. MethodsThe physiological appearance of exosomes isolated from PLC/PRF/5 cells was examined by using differential ultracentrifugation and the images were captured by transmission electron microscopy. The internalization of exosomes by THP-1 cells was examined via laser-scanning confocal microscopy. The expression ofLINC RORwas analyzed through qRT-PCR. The expression of interleukin （IL）-1β was examined by ELISA. ResultsExosomes were found spherical and the diameters mainly distribute around 100 nm.LINC RORcan be transferred by tumor derived exosomes to macrophages. Exosomes isolated from PLC/PRF/5 cells significantly expressed higher levels ofLINC ROR.Suppression ofLINC RORby siRNA significantly increased the release of pro-inflammatory cytokine (IL-1β) in THP-1 cell upon LPS stimulation. ConclusionLINC RORfrom hepatocarcinoma cell-derived exosomes plays an important role in regulating the inflammation of macrophages.
