IL-1β预处理脂肪间充质干细胞促进VEGF蛋白分泌和巨噬细胞M2型极化的研究

Abstract:ObjectiveTo investigate the effects of interleukin (IL)-1β preteated adipose tissue-derived mesenchymal stem cells (ADMSCs) on vascular endothelial growth factor (VEGF) secretion and macrophages M₂polarization. MethodsAfter IL-1β pretreated ADMSC for 24 h,the expression of cyclooxygenase-2 (COX-2) was detected using Western blot,and the secretions of prostaglandin E₂(PGE₂) and VEGF were measured by ELISA method. Conditioned media collected from ADMSCs was used for culturing PMA-U937 macrophages for 72 h,and the expressions ofCD163andIL-10 mRNAs were detected using qRT-PCR. After inhibition of COX-2 expression in ADMSCs by Lentivirus silencing,secretion of VEGF andCD163andIL-10 mRNA expressions were detected. ResultsIL-1β pretreating ADMSCs increased the expression level of COX-2,enhanced PGE₂and VEGF secretions (PCD163andIL-10 were upregulated when ADMSCs were pretreated by IL-1β in PMA-U937 cells. After downregulation of COX-2 in ADMSCs,the secretion of VEGF was suppressed,and the mRNA levels ofCD163andIL-10 were also significantly down-regulated. ConclusionIL-1β pretreatment enhanced the secretion of PGE₂and VEGF,and induced macrophage M₂polarization,which depended on COX-2-PGE₂signal pathway.