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论文摘要

hADMSCs影响TLR4-TRIF信号通路诱导小鼠小胶质细胞表型极化的实验研究

Effect of Human Adipose Mesenchymal Stem Cells on Phenotype Polarization of Mice Microglia via TLR3/TRIF Signal Pathway

作者:孙静, 刘漪沦, 李灿等

Author:SUN Jing, LIU Yi-lun, LI Can.et al

收稿日期:          年卷(期)页码:2019,50(2):164-170

期刊名称:四川大学学报(医学版)

Journal Name:JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION)

关键字:人脂肪间充质干细胞表型Toll样受体

Key words:Human adipose derived mesenchymal stem cells (hADMSCs)PhenotypeToll-like receptors (TLRs)

基金项目:

中文摘要

目的研究人脂肪间充质干细胞(human adipose-derived mesenchymal stem cells,hADMSCs)对小胶质细胞表型极化的影响及机制。方法取 C57/BL6小鼠BV-2小胶质细胞,以 hADMSCs+脂多糖(LPS)间接共培养,或以单纯LPS 培养。倒置显微镜下观察细胞形态。CCK-8法检测小胶质细胞增殖能力,实时荧光定量PCR(RT-qPCR)检测小胶质细胞M1/M2表型标记物的影响,Western blot检测小胶质细胞Toll样受体4(TLR4)-β干扰素TIR结构域衔接蛋白(TRIF)信号通路相关蛋白的表达。结果与单纯LPS培养相比,hADMSCs 加入后,小胶质细胞镜下形态相似,细胞增殖活性被抑制(PPPP

英文摘要

ObjectiveTo explore the effect and mechanism of human adipose-derived mesenchymal stem cells (hADMSCs) on phenotypic polarization of microglia. MethodsBV-2 microglia of C57/BL6 mice were co-cultured with hADMSCs+lipopolysaccharide (LPS),or cultured with LPS alone. Cell morphology was observed under an inverted microscope. The effect of hADMSCs on microglial proliferation was evaluated by CCK-8 assay. The impact of hADMSCs on microglia M1/M2 phenotype markers were detected using quantitative real-time PCR (RT-qPCR). The affect of hADMSCs on the proteins expression levels of Toll-like receptor 4 (TLR4)-TIR domain containing adaptor protein inducing interferon β (TRIF) signaling pathway in BV-2 microglia was detected by using Western blot analysis. ResultsAs compared with the LPS treatment,hADMSCs treatment had no obvious effect on microglia morphology,whereas showed significant inhibition on microglial proliferation activity (PPP

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