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论文摘要

Triton X-100 对Hodge和Carba NP试验检测产碳青霉烯鲍曼不动杆菌复合群效能影响

Triton X-100 Enhanced the Detection of Hodge Test and Carba NP Test for Carbapenemase-producing Acinetobacter baumannii Complex

作者:刘敏雪, 宋启飞, 巫丽娟等

Author:LIU Min-xue, SONG Qi-fei, WU Li-juan. et al

收稿日期:          年卷(期)页码:2018,49(5):771-775

期刊名称:四川大学学报(医学版)

Journal Name:JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION)

关键字:Triton X-100 碳青霉烯酶 鲍曼不动杆菌复合群 Hodge试验 Carba NP试验

Key words:Triton X-100 Carbapenemase A. baumannii complex Hodge test Carba NP test

基金项目:

中文摘要

目的 探索快速、准确、简单、经济的表型检测方法,为产碳青霉烯酶鲍曼不动杆菌复合群流行病学调查及临床诊疗提供更好的手段和依据。方法 纳入四川大学华西医院分离的110株鲍曼不动杆菌复合群,以多重PCR扩增结果为金标准,比较Hodge试验、Carba NP试验与加入Triton X-100后改良的Triton Hodge试验及Carba NP-direct试验在检测鲍曼不动杆菌复合群是否产碳青霉烯酶中的敏感度、特异度及阳性效果。结果 Hodge试验在检测产碳青霉烯酶鲍曼不动杆菌复合群中敏感度和特异度分别为71.1%、100%,高于Carba NP试验(35.0%、86.7%,PPP>0.05)。结论 改良后的Triton Hodge试验及Carba NP-direct试验在检测产碳青霉烯酶鲍曼不动杆菌复合群中具有更高的敏感性及阳性检测判断效果,特异度不变,因此更适用于临床常规操作。

英文摘要

Objective To find out highly effective phenotypic methods to detect carbapenemase-producing Acinetobacter baumannii (A. baumannii complex) so as to support the epidemiological investigation and clinical application. Methods We included 113 A. baumannii complex and compared the detection performance of modified Hodge test, Carba NP test, Triton Hodge test, and the simplified Carba NP-direct test with Tritont X-100. Results We tested 83 carbapenemase-producing A. baumannii complex and 30 non-carbapenemase producers. The sensitivity and specificity of Hodge test were significantly higher than those of Carba NP test (71.1% versus 35.0%,100% versus 86.7%,PPP>0.05). The positive additive effects of the two methods with Triton X-100 were more obvious than those of the methods without Triton X-100 (PA. baumannii complex. Triton Hodge test and Carba NP-direct test were more applicable for clinical routine procedure.

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