Objective To develop a questionnaire measuring sexual mental health of Tibetan university students. Methods A draft questionnaire was developed with reference to the Sexual Civilization Survey for University Students of New Century and other published literature, and in consultation with experts. The questionnaire was tested in 230 students. Exploratory factor analyses with principal component and varimax orthogonal rotation were performed. Common factors with a >1 eigenvalues and ≥3 loaded items (factor loading ≥0.4) were retained. Items with a α and split-half reliabilities were estimated. Confirmatory factor analyses were performed to test the construct validity of the questionnaire. Results Four rounds of exploratory factor analyses reduced the draft questionnaire items from 39 to 34 with a 7-factor structure. The questionnaire had a Cronbach’sα of 0.920, 0.898, 0.812, 0.844, 0.787, 0.684, 0.703, and 0.608, and a Spearman-Brown coefficient of 0.763, 0.867, 0.742, 0.838, 0.746, 0.822, 0.677, and 0.564 for the overall questionnaire and its 7 domains, respectively, suggesting good internal reliability. The structural equation of confirmatory factor analysis fitted well with the raw data: fit index χ2/df=3.736; root mean square residual (RMR) =0.081; root mean square error of approximation (RMSEA)=0.076; goodness of fit index (GFI)=0.805; adjusted goodness of fit index (AGFI)=0.770; normed fit index (NFI)=0.774; relative fit index (RFI)=0.749; incremental fit index (IFI)=0.824; non-normed fit index (NNFI )=0.803; comparative fit index (CFI)=0.823; parsimony goodness of fit index (PGFI)=0.684; parsimony normed fit index (PNFI)=0.698; parsimony comparative fit index (PCFI)=0.742, suggesting good construct validity of the questionnaire. Conclusion The Sexual Mental Health Questionnaire for Tibetan University Student has demonstrated good reliability and validity.