β-葡聚糖生物强化大米急性毒性和遗传毒性评价

ObjectiveTo provide a scientific evaluation of the food safety of the rice biofortified with β-glucan.MethodsThe acute toxicity and genotoxicity of the rice were evaluated by 14-day feeding experiment, Ames experiment, erythrocyte micronucleus test and mouse lymphoma thymidine kinase gene (TK) mutation assay respectively.ResultsIn the acute toxicity test, there was no obvious toxicity of rice biofortified with β-glucan, and no abnormality was found in anatomical observation. The median lethal dose (LD₅₀) to rats and mice wereall greater than 15 mg/kg, which belonged to the actual non-toxic level. Whether with S₉activation or not, no genotoxicity was found to the tested strains TA97a, TA98, TA100, TA102 and TA1535. No induction of polychromatic erythrocytes and inhibition of bone marrow were found in erythrocyte micronucleus test. The results ofTKgene mutation assay did not show the mutagenicity of β-glucan bioaugmentation rice. All results of the three genotoxicity tests were negative.ConclusionUnder the current experimental conditions, β-glucan biofortified rice showed no obvious acute toxicity and genotoxicity.