不同分化程度的口腔鳞状细胞癌组织中蛋白激酶D的表达
Expression Level of Protein Kinase D in Oral Squamous Cell Carcinoma with Diverse Differentiation
作者：陈红利, 崔博淼, 康颖竹, 王利伟, 王京楠, 冯云, 张平, 吕蝶, 陈娇
Author：CHEN Hong-li, CUI Bo-miao, KANG Ying-zhu, WANG Li-wei, WANG Jing-nan, FENG Yun, ZHANG Ping, LÜ Die, CHEN Jiao
收稿日期：2019-11-14 年卷（期）页码：2020,51(6):755-759
期刊名称：四川大学学报（医学版）
Journal Name：JOURNAL OF SICHUAN UNIVERSITY （MEDICAL SCIENCE EDITION）
关键字：口腔鳞状细胞癌, 蛋白激酶D, 细胞分化, 相关性分析
Key words：Oral squamous cell carcinoma, Protein kinase D, Cell differentiation, Correlation analysis
基金项目：
中文摘要
目的 探索口腔鳞状细胞癌（oral squamous cell carcinoma, OSCC）组织中蛋白激酶D（protein kinase D, PKD）1、2、3的表达情况及其与口腔鳞癌分化程度的关系。 方法 收集10例正常口腔上皮组织、40例OSCC组织样本，采用免疫组化SP法检测PKD1、PKD2和PKD3的表达，评判阳性细胞比率及对染色强度进行评分，分析不同分化程度的OSCC组织中PKD1、PKD2和PKD3间的差异性，并分析PKD1、PKD2和PKD3免疫组化染色评分与OSCC分化程度的相关性。 结果 PKD1和PKD3在OSCC组织中高表达；不同分化程度的OSCC组织中，PKD1、PKD2及PKD3免疫组化染色评分间差异均有统计学意义（P<0.001）；PKD1和PKD2的免疫组化染色评分与OSCC分化程度呈负相关关系（r分别为−0.574和−0.341, P<0.001）。 结论 不同分化程度的OSCC组织中，起主导作用的PKDs可能存在差异；PKD1和PKD2的表达水平与OSCC的分化程度存在相关性，OSCC分化程度越低，PKD1及PKD2的表达越高。
英文摘要
ObjectiveThis study aimed to investigate the expression level of protein kinase D (PKD) in oral squamous cell carcinoma (OSCC) and its relationship with differentiation of OSCC.MethodsSample was collected from 10 healthy control subjects and 40 OSCC confirmed by histopathological diagnosis, and the immunohistochemical staining was adopted to detect the expression of PKDs in OSCC tissues. The proportion of stained cell and staining intensity were evaluated to get a score, which used to analyze the difference among PKD1, PKD2 and PKD3 in various differentiation OSCC tissues. The correlations between the staining score of PKDs and differentiation of OSCC were further analyzed.ResultsPKD1 and PKD3 were high expression in OSCC tissues. There were statistical significance among the staining score of PKD1, PKD2 and PKD3 in various differentiation OSCC tissues (P<0 .001). in addition, there was a significantly negative correlation between the staining score of pkd1 and pkd2 with the differentiation of oscc (r=−0.574, −0.341,P<0 .001).ConclusionIn OSCC tissues with different degree of differentiation, there might be some differences among PKDs which play a major role. The expression of PKD1 and PKD2 was correlated with the differentiation of OSCC, the poor differentiation of OSCC, the higher expression of PKD1 and PKD2.
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