黄连素与戊间甲酚对粪肠球菌的体外协同抗菌效果研究

ObjectiveTo study the antibacterial effect of berberine combined with amylmetacresol onEnterococcus faecalis.MethodsBoth dilution method and live bacteria CFU were used to determine the minimum inhibitory concentration (MIC) of berberine and amylmetacresol onE. faecalis. The killing effect of berberine and amylmetacresol on planktonicE. faecaliswas detected by suspension quantitative germicidal test and live/dead bacteria staining. The effects of berberine and amylmetacresol on the structure of mature biofilm ofE. faecaliswas observed by scanning electron microscopy (SEM). The toxicity of berberine and amylmetacresol on human oral keratinocytes (HOK) was determined by CCK-8 cell proliferation and cytotoxicity assay and cytotoxicity LDH assay.ResultsThe MIC of berberine was 512 μg/mL, and the MIC of amylmetacresol was 0.023 3%. 512 μg/mL berberine and 0.002 33% amylmetacresol had a weak killing effect on planktonicE. faecalisalone, while they showed a synergistic antibacterial effect in combination. Cell survival in the biofilm was only slightly changed by berberine and amylmetacresol. The structure of biofilm was obviously changed by berberine and amylmetacresol. 512 μg/mL berberine and 0.002 33% amylmetacresol alone or in combination showed the survival rate was much higher than the injury rate, suggesting berberine and amylmetacresol had a low cytotoxicity.ConclusionBerberine and amylmetacresol had synergism againstE. faecalis, and the biological safety of the combination use was better.