Previous fatigue tests on the anchorage zones, the orthotropic steel decks and the suspenders of suspension bridges are only the experiments of single component. But the stresses of these components are very complex and in a complicated spatial stress states, and the boundary conditions on the model have the great impact on fatigue test results. To evaluating the fatigue performance on the key structural details of Qingcaobei Yangtze River bridge, and verifying the rationality of the design, safety and durability of the bridge, a 1:4 scaled integration model fatigue test including main cables, anchorage zones, hangers and box girders is carried out. Experiment results show that, the stresses redistribution in the integrated model after fatigue loading is not obvious; the principal stresses of static loads test during the fatigue experiment are small different; the relationship between displacement and load is approximately linear. Displacement and each static load test values in the process of loading and unloading has good symmetry and recoverability, which shows that the whole structure is in the elastic stage. Fatigue cracks are not found in the key structure details the structure under the design of fatigue loading, so the structural fatigue performance meet the design requirements. Through the study of the fatigue experiment of integrated model structure, the fatigue performance of the integrated model can reflect the actual fatigue performance of the cable-anchorage -hanger-girder structure, and the integrated fatigue experiment can provide some certain reference significance.