This paper presents a finite element analysis on flexural behaviors of box girders with corrugated steel web (hereafter called box girders). Displacement, stress and strain under static load is used to confirm the failure mechanism and stress weak spots. Meanwhile, fatigue life of box girders in different conditions is predicted by analyzing local stress and strain action, which is acquired by finite element analysis. Based on the fatigue damage and fatigue fracture theory, the predicted results of cracks initiation location and fatigue life are approximately agree with the experimental. Results show that the standard C of America code AASHTO2004 is suitable for designing the fatigue life of box girders, What is more, the Palmgren-Miner learner accumulated damage criterion can be an efficient method to analysis the fatigue damage process, which provides a reference for box girders fatigue design in practical engineering.