Based on the multi-body system kinematics theory, the error modeling of three-axis simulation turntable is established, and the basic kinematics law of the turntable with error is discussed. According to the structure and principle of turntable, all the turntable assembling errors’ effects on the orientation error and position error of the turntable are analyzed in detail. With the computer simulation, the influence of all error factors on the precision of the turntable’s orientation and location is compared. These researches also can offer reference to error allocation and error probability of the simulation experiment to improve the experimental accuracy.