Strain GCN019 with high ability to degrade beta-cypermethrin was isolated from garden soil which contaminated by beta-cypermethrin for a long term.The strain was identified asPseudomonas fluorescensbased on the results from its physiological-biochemical characteristics and phylogenetic similarity of 16S rDNA gene sequences.Then,the main factors influencing beta-cypermethrin degradation were investigated by using Plackett-Burman design,and the degradation condition was optimized with response surface methodology.It was found that beta-cypermethrin concentration,inoculum volume and initial pH value were the major factors influencing beta-cypermethrin degradation.In addition,the degradation rate of beta-cypermethrin under the optimized condition reached 73.94%,which was similar to the predictive value from response surface model(74.30%).
