Startup experiments of hydrogen bioproduction CST reactor were conducted. The molasses was used as substrate and different types of seed sludge were utilized. The results indicated that initial microbe community is one of the crucial factors on reactor’s startup speed. At medium temperature 35±1℃, hydraulic retain time（HRT）about 9h, organic loading rate (OLR) between 8~10kgCOD/m3﹒d, aerobic activate sludge, anoxic activate sludge and anaerobic activate sludge can be used as seed sludge. The startup times are 35d, 25d and more than 50d respectively. Anoxic activate sludge is the optimal seed sludge for rapid startup of hydrogen bioproduction CST reactor.
