Abstract：A novel acclimation of “independent”and “on-line” was developed to study the power generation performance of this acclimate mode with three anodic materials including carbon felt, carbon cloth and carbon paper. The experimental results showed that the anodic characters such as the surface area, aperture distribution, surface roughness and surface electric potential had directly affected the biomass on anode in the period of “independent acclimation”. It was clear that the anodic materials were in turn carbon felt, carbon cloth and carbon paper for abundant biomass on it by SEM. After about 10h in “on-line acclimation”, the electrocatalytic activity of bacteria catalyzer was increased significantly. In the second cycle, the output voltages for MFCs reached the peak (carbon felt/0.803V; carbon cloth/0.604V; carbon paper/0.574V). In the third cycle, MFCs maintained a high stable running for long period, especially the carbon felt-biofilm，the maximal output voltage (0.78V) of it could keep more than 180h. Comparing with power density of MFCs, the priority was in turn the carbon felt (1339.6 mW/m3), carbon paper (96.8mW/m3) and carbon cloth (80mW/m3). The efficiencies of COD removal were in turn carbon felt (89.5%), carbon paper (79%) and carbon cloth (74.7%). The main merits of the acclimation were convenient operations and high electrical functions.
