The biodegradation of wastewater from sugar industry in a three phase biological fluidized reactor was investigated. The reactor was continuously fed with wastewaters from different workshop sections. The results demonstrated that the average CODCrremoval efficiency was above 85%. When the BOD/CODCr(B/C) of the wastewater is above 0.5, the effluent CODCris below 80 mg/L and when the B/C is 0.4,CODCr in effluent is steadily around 110 mg/L. The performance of biodegradation of nitrogen was proved to be effective and the reduction rate of NH3-N is around 80%. Meanwhile, the resistance capability of the reactor to impact load and the RTD of the reactor were investigated through experiments. Based on the analysis of the results, the reactor was resisted well with the impact variance of the CODCr load in the influent. The capability of resistance to impact load comes from, on one side, the immobilization of biofilms, for another, the dilution effect of liquid due to the sufficient mixing extent in the reactor.