The aim of route optimization in Mobile IPv6 is to shorten the end-to-end delay between the mobile node and the correspondent node, but the impact of route optimization on the network performance is still unclear. The NS-2, a network simulation software,was used to create a fairly complicated Mobile IPv6 network environment for observing the impact of the route optimization on some typical Mobile IPv6 network performance parameters, such as handoff latency, packet loss and end-to-end delay. By analyzing the simulation results, an advanced formulas of handoff latency and packet loss was put forward,and the results showed that the route optimization may increase the handoff latency and packet loss.