An important problem before the parameters identification of biological inverse problems, that is, the determination of the position of measuring points,was discussed.The Bayesian theory was applied to define the probability of the samples by the posterior probability of the undetermined parameter and the information entropy was used to describe the measured information.The “contribution” of different measuring points was expressed by the evaluation factor that was defined by information entropy.The calculation result of single species biological model showed that the tendency of reconstruction error of the undetermined parameter can be reflected by the evaluation factor that could also describe the contribution of every measuring area.