不同马铃薯基因型对根际细菌群落结构的影响
Effects of potato genotype on rhizosphere bacterial community structure
作者：颜朗(西昌学院农业科学学院， 四川马铃薯高等学校重点实验室)；张义正(四川大学生命科学学院 四川省分子生物学及生物技术重点实验室)；方志荣(西昌学院农业科学学院， 四川马铃薯高等学校重点实验室)；清源(西昌学院农业科学学院， 四川马铃薯高等学校重点实验室)；黄莓(西昌学院农业科学学院， 四川马铃薯高等学校重点实验室)；赖先军(西昌学院农业科学学院， 四川马铃薯高等学校重点实验室)
Author：YAN Lang(College of Agriculture Science, Xichang College, Sichuan Key Laboratory of Potatoes)；ZHANG Yi-Zheng(Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Sciences, Sichuan University)；FANG Zhi-Rong(College of Agriculture Science, Xichang College, Sichuan Key Laboratory of Potatoes)；QING Yuan(College of Agriculture Science, Xichang College, Sichuan Key Laboratory of Potatoes)；HUANG Mei(College of Agriculture Science, Xichang College, Sichuan Key Laboratory of Potatoes)；LAI Xian-Jun(College of Agriculture Science, Xichang College, Sichuan Key Laboratory of Potatoes)
收稿日期：2019-05-22 年卷（期）页码：2020,57(2):383-390
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：马铃薯； 根际微生物； 群落多样性； 高通量测序； 高山地方种
Key words：Potato; Rhizosphere microbe; Community diversity; High throughput sequencing; Alpine landraces
基金项目：四川省科技厅应用基础项目（2019YJ0546）; 四川省教育厅重大培育项目（50160001）; 西昌学院高层次人才引进科研启动项目（50180108，50190005）; 西昌学院博士启动基金（2017BS12）
中文摘要
本研究旨在从根际细菌群落结构的角度阐述不同马铃薯基因型对根际细菌菌群的影响，探讨高山地方种根际细菌菌群特性，挖掘可能对地方种定殖起关键作用的细菌类型.本研究通过高通量测序数据对不同马铃薯基因型根际细菌16S rRNA基因进行操作分类单位（OTU）聚类，分析样品间细菌群落的多样性特征，并通过OTU丰度差异分析揭示引起基因型效应的根际细菌类型.测序结果经质量控制，共获得3 097 269条高质量序列（Clean tags）并依据97%的序列相似性聚类到1 565个有效OTUs，品种间OTU种类相差不大.群落多样性反映高山地方种乌洋芋和牛角洋芋根际细菌群落较其他基因型存在着显著差异.乌洋芋和主栽品种米拉间OTU丰度分析表明乌洋芋中55个OTU丰度上调，143个丰度下调，差异显著.本研究表明，马铃薯根际细菌群落多样性受马铃薯基因型因素的影响，高山地方种相较于自育种和主栽种其根际细菌群落结构表现出显著的差异.
英文摘要
This study aims to elucidate the effects of different potato genotypes on rhizosphere microbial communities from the perspective of rhizosphere bacterial community structure, and to explore the characteristics of rhizosphere bacterial communities in high-altitude landraces and to identify the bacterial category playing an important role in colonization. The high-throughput sequencing of rhizosphere bacterial 16S rRNA genes was used to cluster the operational taxonomic units (OTUs) and the diversity characteristics of bacterial communities. We also used differential analysis of the abundance of OTU to identify the category of rhizosphere microorganism related to the genotypic effect. The sequencing results were quality controlled, and a total of 3 097 269 clean tags were obtained and clustered to 1 565 effective OTUs based on 97% sequence similarity. Overall, the bacterial category across different cultivars was almost the same, while the high-altitude landraces Purple potatoes and Horn potatoes showed a significant difference in the diversity of the rhizosphere microbial community. The abundance of OTU analysis between purple potatoes and the main variety Mira showed that the abundance of 55 OTUs in purple potatoes was significantly up-regulated and 143 was down-regulated. This study demonstrated that the diversity of potato rhizosphere bacterial community was affected by potato genotype factors, and the rhizosphere bacterial community structure of alpine landraces showed significant differences compared with local-breeding and main varieties.
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