枸杞抗坏血酸氧化酶基因的克隆与表达分析

Molecular Cloning and Expression Patterns of LcAO from Lycium Chinens

作者：乔枫(青海师范大学青藏高原资源与环境教育部重点实验室)；耿贵工(青海省农林科学院农业部农产品质量安全风险评估实验室)；谢惠春(青海师范大学青藏高原资源与环境教育部重点实验室)

Author：QIAO Feng(Educational Ministry Lab of Environment and Resource in Qinghai-Tibet Platean, Qinghai Normal University)；GENG Gui-Gong(Laboratory of Quality & Safety Risk Assessment for Agro-products, Ministry of Agriculture)；XIE Hui-Chun(Educational Ministry Lab of Environment and Resource in Qinghai-Tibet Platean, Qinghai Normal University)

收稿日期：2014-11-18 年卷（期）页码：2016,53(1):203-208

期刊名称：四川大学学报: 自然科学版

Journal Name：Journal of Sichuan University (Natural Science Edition)

关键字：枸杞；抗坏血酸氧化酶 (AO)；基因克隆；荧光定量PCR

Key words：Lycium Chinense；ascorbate oxidase（AO）；gene cloning；quantitative real-time PCR

基金项目：

中文摘要

以枸杞为材料，利用同源克隆技术，克隆了枸杞抗坏血酸氧化酶（AO）的全长 cDNA序列，命名为 LcAO 基因（GenBank：KP712033），该基因开放阅读框（ORF）大小为1737 bp，编码 578 个氨基酸，与西红柿 AO 蛋白的同源性达到 90％。LcAO 编码的氨基酸序列包含 3 个多铜氧化酶结构域和跨膜信号序列。采用实时荧光定量 PCR 分析显示，LcAO 基因在枸杞的花和果实中表达量最高，成熟叶中表达量最少。

英文摘要

A ascorbate oxidase (AO) gene from Lycium Chinense was cloned with homologous method. The full-length cDNA of Lycium Chinense AO which was named LcAO (Genbank: KP712033) was 1737 bp，and contained a complete open reading frame (ORF) of 578 amino acid residues. Homology analysis indicated that the deduced LcAO protein was highly 90% to AO proteins from Solanum lycopersicum. The results from Real-Time PCR indicated that the expression of LcAO gene was the strongest in the flower and friut, and the least in mature-leaf of Lycium Chinense.

上一条：人翻译延伸因子eEF1A1促进肺癌细胞H1299对紫杉醇和阿霉素药物耐受的研究

【关闭】

论文摘要

枸杞抗坏血酸氧化酶基因的克隆与表达分析

Molecular Cloning and Expression Patterns of LcAO from Lycium Chinens