麻疯树基因JcKNOX1的克隆和表达调控分析
Cloning and expression regulation analysis of a JcKNOX1 gene, from Jatropha curcas L.
作者：时小东(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；田银帅(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；闫晓雪(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；方志荣(1.四川大学生命科学学院生物资源与生态环境教育部重点实验室;2.西昌学院动物科学学院)；王胜华(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；陈放(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：SHI Xiao-Dong(Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；TIAN Yin-Shuai(Key Laboratory of Bio-source and Eco-environment of Ministry of Education，College of Life Sciences,Sichuan University)；YAN Xiao-Xue(Key Laboratory of Bio-source and Eco-environment of Ministry of Education，College of Life Sciences，Sichuan University)；FANG Zhi-Rong(1.Key Laboratory of Bio-source and Eco-environment of Ministry of Education，College of Life Sciences，Sichuan University;2．School of Animal Science of Xichang College)；WANG Sheng-Hua(Key Laboratory of Bio-source and Eco-environment of Ministry of Education，College of Life Sciences，Sichuan University)；CHEN Fang(Key Laboratory of Bio-source and Eco-environment of Ministry of Education，College of Life Sciences，Sichuan University)
收稿日期：2014-09-06 年卷（期）页码：2016,53(1):187-192
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：麻疯树；JcKNOX1；序列分析；表达分析
Key words：Jatropha curcas L；JcKNOX1；Sequence analysis；Expression analysis
基金项目：国家“十二五”重大科技支撑计划课题（2011BAD22B08）；四川省教育厅项目（08ZB015）
中文摘要
KNOX同源异型盒基因在植物生长发育中扮演着极其重要的角色，首次从大戟科植物麻疯树cDNA中克隆到一个同源异型盒基因，命名为JcKNOX1.其开放阅读框全长693 bp，编码230个氨基酸残基，预测蛋白质等电点 pI=6.13，分子量 PA=25.92 KD．聚类分析表明，JcKNOX1与毛白杨在进化上具有最近的亲缘关系，其次是甜橙．荧光定量PCR检测发现，JcKNOX1基因在麻疯树植株各个器官中均有表达，幼嫩部位表达量明显高于成熟器官，表达量依次为：茎尖＞嫩叶＞茎＞叶＞花＞叶柄＞根；对麻疯树幼苗进行ABA和低温胁迫发现，JcKNOX基因表达量明显增加．上游调控元件预测分析表明，该基因可能在麻疯树光调节、激素调控、花粉基因特异性表达、逆境调控等重要生物学过程中具有重要作用．
英文摘要
KNOX family genes，coding homeobox proteins，play an inmportant role in plant development．A novel homeobox gene，JcKNOX1，was cloned from the cDNA of Jatropha curcas L.．The full-length ORF of JcKNOX1 was 693bp，encoding a protein of 230 amino acids residures．The predicted structure of JcKNOX1 showed that its theoretical isoelectric point and molecular weight were 6.13 and 25.92 kDa respectively．Phylogenic analysis indicated that JcKNOX1 was relation to Populus tomentosa and Citrus sinensis closely．By fluorescence quantitative PCR the expression levels were different as follows：stem apex>tender leaf>stem>leaf>flower>petiole>root，which showed that the JcKNOX1 expressed with high levels in developing organs．Meanwhile，JcKNOX was strongly induced by ABA and low temperature in Jatropha curcas L. seedlings．Through biology software prediction analysis indicated that JcKNOX1 might play an important role in light regulation，hormone regulation，expression of pollen-specific gene and abiotic stress of Jatropha curcas L.．
上一条：山桐子油的提取分离及理化性质研究
【关闭】
