藏红花花瓣粗多糖的体外抗氧化活性研究
The antioxidant activity in vitro of crude polysaccharide from Crocus sativus petals
作者：陈可(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；曲绮雯(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；陈霞连(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；白洁(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；陈放(四川大学生命科学学院生物资源与生态环境教育部重点实验室)
Author：CHEN Ke(Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；QU Qi-Wen(Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；CHEN Xia-Lian(Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；BAI Jie(Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)；CHEN Fang(Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University)
收稿日期：2014-12-26 年卷（期）页码：2016,53(2):448-452
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：藏红花；多糖；抗氧化活性
Key words：Crocus sativus, polysaccharide, antioxidant activity
基金项目：西红花规模化生产技术服务（HS20100601105584）；四川省科技厅应用基础项目（2012JY0090）；四川大学植物标本数字化平台建设及共享（2012JCPT004）
中文摘要
采用水提醇沉法提取藏红花花瓣中的多糖，得率为3.75%。联合蒽酮-硫酸法和DNS法测得多糖纯度为68.34%。对粗多糖的体外抗氧化活性进行测定，结果表明，粗多糖具有一定的抗氧化活性，抗氧化能力指数（ORAC）值为0.572 μmol TE/mg；其对ABTS+、DPPH和羟自由基具有一定的清除能力，半数清除浓度EC50分别为76.26 μg/mL、165.5 μg/mL和880.4 μg/mL。
英文摘要
The yield of polysaccharide from Crocus sativus petals was 3.75% by water extraction and alcohol precipitation. The purity of crude polysaccharide was 68.34%, measured by anthrone-sulfuric acid method and DNS method. The antioxidant activity in vitro of crude polysaccharide was also determined. The results showed that crude polysaccharide exhibited certain antioxidant activity with the ORAC value of 0.572 μmol TE/mg, and had effect on scavenging ABTS+, DPPH and hydroxyl radical with the half elimination concentration (EC50) of 76.26 μg/mL, 165.5 μg/mL and 880.4 μg/mL respectively.
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