A型口蹄疫病毒（FMDV）VP1基因遗传进化分析
The Phylogenetic Analysis of Foot-and-mouth Disease Virus (FMDV) Serotype A Based on VP1 Gene
作者：杨江(四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, “985工程”西南资源环境与灾害防治科技创新平台)；范辰韵(四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, “985工程”西南资源环境与灾害防治科技创新平台)；高文倩(四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, “985工程”西南资源环境与灾害防治科技创新平台)；罗晓林(四川省草原科学研究院)；安添午(四川省草原科学研究院)；庞倩(四川省草原科学研究院)；许路来(四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, “985工程”西南资源环境与灾害防治科技创新平台)；徐岳松(四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, “985工程”西南资源环境与灾害防治科技创新平台)；徐鹏卫(四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, “985工程”西南资源环境与灾害防治科技创新平台)；杨鑫(四川大学生命科学学院, 动物疫病与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, “985工程”西南资源环境与灾害防治科技创新平台)
Author：YANG Jiang(Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University)；FAN Chen-Yun(Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University)；GAO Wen-Qian(Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University)；LUO Xiao-Lin(Sichuan Academy of Grassland Science)；AN Tian-Wu(Sichuan Academy of Grassland Science)；PANG Qian(Sichuan Academy of Grassland Science)；XU Lu-Lai(Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University)；XU Yue-Song(Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University)；XU Peng-Wei(Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University)；YANG Xin(Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education. "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern. College of Life Sciences, Sichuan University)
收稿日期：2014-11-27 年卷（期）页码：2016,53(2):430-436
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：口蹄疫病毒；VP1；A型；进化树分析
Key words：Foot-and-mouth disease virus; VP1; Serotype A; Phylogenetic analysis
基金项目：四川省科技支撑计划“牦牛现代产业链关键技术集成研究与产业化示范”（2012NZ0047）；四川省科技基础条件平台项目（14010136）；国家自然科学基金项目（J1103518）
中文摘要
对疑似感染FMDV的样本进行了FMDV VP1基因 RT-PCR鉴定，测定了其中1个阳性样本序列（命名为HY）。采用生物信息学软件比较了HY与参考序列的同源性，并构建VP1基因的分子系统树。结果表明：HY属A型FMDV亚洲拓扑型东南亚-97谱系，与我国近年来报道的A/GDMM/CHA/2013的序列相似性为99.1%，与AF/72疫苗株的相似性仅为79.6%。提示我国近年来报道的A型毒株与AF/72疫苗株的序列差异较大，应及时更换A型口蹄疫疫苗毒株。
英文摘要
The samples suspected infection of FMDV were identified by RT-PCR method based on VP1 gene. One of the positive samples was sequenced and applied to homologous analysis with other reference sequences, to construct phylogenetic tree of VP1 gene. The results showed that the isolated VP1 belonged to serotype A, Asia topological type, and Sea-97 lineage. It has a high sequence similarity (99.1%) to A/GDMM/CHA/2013, a Chinese A type FMDV isolated in 2013, whereas has a low sequence similarity to the vaccine strain AF/72 (79.6%). The low sequence simi-larity between vaccine strain AF/72 and the A-type FMDV viruses reported recently indicated that AF/72 vaccine strain might only provide limited protection and the vaccine strain should be made up to date as soon as possible.
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