Pin1的细胞定位调控Rb蛋白的磷酸化状态
Cellular localization of Pin1 regulates the phosphorylation status of Rb
作者：刘瑞红(四川大学生命科学学院生长代谢与衰老研究中心/生物资源和生态环境教育部重点实验室)；谢刚(四川省中西医结合医院)；肖智雄(四川大学生命科学学院生长代谢与衰老研究中心/生物资源和生态环境教育部重点实验室)
Author：LIU Rui-Hong(Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment of Ministry of Education, College of Life Sciences, Sichuan University)；XIE Gang(Sichuan Integrative Medicine Hospital)；XIAO Zhi-Xiong(Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment of Ministry of Education, College of Life Sciences, Sichuan University)
收稿日期：2014-10-12 年卷（期）页码：2016,53(2):419-423
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：Pin1 视网膜母细胞瘤蛋白 细胞定位 磷酸化
Key words：Pin1, Retinoblastoma protein, cellular localization, phosphorylation
基金项目：国家自然科学基金31171362
中文摘要
视网膜母细胞瘤蛋白(Rb)在调控细胞增殖和肿瘤的发生发展中起着至关重要的作用。最新的研究结果表明，蛋白异构酶(Pin1)可调控Rb的功能。我们对Pin1和Rb蛋白的细胞定位、Pin1调控Rb磷酸化的相关机制进行了研究。结果表明，沉默Pin1可降低Rb的磷酸化并抑制细胞的增殖；培养的肿瘤细胞和肿瘤组织中，Pin1可定位到细胞质中，而胞质定位的Pin1也可增加Rb的磷酸化。这是Pin1调控Rb功能的一个崭新的方式。
英文摘要
Retinoblastoma tumor-suppressor proteins (Rb) plays a vital role in regulation of cell proliferation and tumor development. Recent research reveals that peptidyl-prolyl isomerase (Pin1) can regulate the function of Rb. In this study, we examined the cellular localization of Pin1 in tumor tissues, and investigated the effects of Pin1 localization on Rb phosphorylation. Our data demonstrated that knockdown of Pin1 result in a decrease of Rb phosphorylation and cellular viability. Pin1 can localize to cytoplasm in cultured tumor cells and tumor tissues, while cytoplasmic Pin1 can increase the phosphorylation of Rb. This is a novel mechanism for Pin1 in regulating Rb function.
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