Growth hormone-releasing hormone (GHRH) receptor (GHRHR) belongs to the G protein-coupled receptor (GPCR) B-I subfamily, which is capable of mediating the actions of GHRH on pituitary growth hormone synthesis and release. In the study, we constructed the prokaryotic expression plasmids encoding the N-terminal fragment of chicken GHRHR1 and GHRHR2 and induced their expression in E. coli by IPTG treatment. Moreover, the recombinant GHRHR1 and GHRHR2 fusion proteins were purified and used to prepare the antibodies against chicken GHRHR1 and GHRHR2 in rabbits. In addition, the specificity of the antibodies against chicken GHRHRs was evaluated by Western blot analysis. The results from this study pave the way for further investigation of the localization and roles of GHRHRs in chicken tissues.
