重庆南川区道地药材玄参遗传多样性ISSR分析
Genetic diversity of trueborn Scrophularia ningpoensis Hemsl in Nanchuan district, Chongqing revealed by ISSR analysis
作者：马腾蛟(四川大学生命科学学院遗传医学研究所, 特色生物资源研究与利用川渝共建重点实验室)；陈杰(四川大学生命科学学院遗传医学研究所, 特色生物资源研究与利用川渝共建重点实验室)；丁显平(四川大学生命科学学院遗传医学研究所, 特色生物资源研究与利用川渝共建重点实验室; 重庆市南川生物技术研究院)；陈鸿翰(四川大学生命科学学院遗传医学研究所, 特色生物资源研究与利用川渝共建重点实验室)；包善飞(四川大学生命科学学院遗传医学研究所, 特色生物资源研究与利用川渝共建重点实验室; 重庆市南川生物技术研究院)
Author：MA Teng-Jiao(College of Life Sciences, Sichuan University, Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics)；CHEN Jie(College of Life Sciences, Sichuan University, Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics)；DING Xian-Ping(College of Life Sciences, Sichuan University, Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics; Biotechnology academy of Nanchuan)；CHEN Hong-Han(College of Life Sciences, Sichuan University, Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics)；BAO Shan-Fei(College of Life Sciences, Sichuan University, Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Institute of Medical Genetics; Biotechnology academy of Nanchuan)
收稿日期：2015-05-05 年卷（期）页码：2016,53(5):1119-1124
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：玄参；种植中药材；ISSR ；遗传多样性
Key words：Scrophularia ningpoensis Hemsl; Cultural Chinese medicine herb; Inter-simple sequence repeat (ISSR); Genetic diversity
基金项目：国家工信部重大专项
中文摘要
本研究利用ISSR-PCR分子标记技术分析研究了重庆市南川区五个地方人工种植玄参的遗传多样性，利用7条UBC引物共扩增出了61条DNA片段，其中多态性片段为48条。片段长度约为250～2000 bp，每条引物扩增出的DNA片段数为4～14条。同时采用NTSYS-pc2.1软件计算出五个区域之间和区域内部子区域的玄参的Nei’s遗传距离，并用 UPGMA法分别构建了系统树。结果表明五个地方人工种植玄参存在一定的遗传多态性，但其地方之间差异并不明显，其遗传多样性主要可在同一地方内的子区域间被观察到。
英文摘要
Genetic diversity of trueborn Scrophularia ningpoensis Hemsl in five areas of Nanchuan district, Chongqing was preliminary analyzed using inter-simple sequence repeat (ISSR) marker. Seven UBC primers were used in this research, and a total number of 61 DNA fragments were obtained, with 48 were polymorphic in total. The range of the numbers of DNA fragments amplified by each primer was 4 to 14. The Nei’s genetic distances of the five areas and their subareas were calculated using NTSYS-pc2.1 software and the dendrogram were constructed through UPGMA method. The results revealed that the genetic diversity of Scrophularia ningpoensis Hemsl cultivated separately in those five areas could be observed, but it was not obvious among the five big areas but their subareas.
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