中国西南主要苹果产区潜隐性病毒分子鉴定
The molecular identification of latent viruses in the main apple production areas in Southwest China
作者：姚润东(四川大学生命科学学院生物资源与生态环境教育部重点实验室)；史文森(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；孙吴润泽(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；黄奎(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；王健美(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)
Author：YAO Run-Dong(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University)；SHI Wen-Sen(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University)；UNWU Run-Ze(Key Laboratory of Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；HUANG Kui(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University)；WANG Jian-Mei(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University)
收稿日期：2018-02-12 年卷（期）页码：2019,56(2):357-362
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：潜隐性病毒; 苹果茎痘病毒; 苹果茎沟病毒; 苹果褪绿叶斑病毒; 逆转录聚合酶链反应
Key words：Latent virus; ASPV; ASGV; ACLSV; RT-PCR
基金项目：国家转基因重大专项（2016ZX08009003-002）
中文摘要
为了深入了解苹果受潜隐性病毒的影响情况, 本研究针对云南、贵州、四川三省的387份苹果叶片样本,利用RT-PCR及多重RT PCR技术,分析鉴定了三种苹果潜隐性病毒——苹果茎痘病毒（ASPV）、苹果茎沟病毒（ASGV）和苹果褪绿叶斑病毒（ACLSV）在苹果上的发生现状.结果显示,苹果样本中ASPV阳性率为89.4%,ASGV阳性率为100%,ACLSV阳性率为81.6%,且它们多为混合感染,共计304份样本同时感染了三种病毒,混合感染率为 78.5% .与云南、贵州相比,采自四川的苹果样本受潜隐性病毒感染最为严重.本研究报道了我国西南苹果主产区潜隐性病毒感染现状,建立了一套快速有效的苹果潜隐性病毒分子生物学鉴定方法.
英文摘要
In order to gain a further study of the impact of apples on the latent virus, 387 samples of apple leaves from the provinces of Yunnan, Sichuan and Guizhou were collected in this study. Three kinds of apple latent viruses, including ASPV（Apple stem pitting virus）, ASGV (Apple stem grooving virus) and ACLSV (Apple chlorotic leaf spot virus), were analyzed and identified by RT-PCR (Reverse Transcription PCR) and multiplex RT-PCR. The results demonstrated that the incidence rate of ASPV, ASGV, and ACLSV was 89.4%, 100%, and 81.6%, respectively. In addition, 304 out of 387 samples were co-infected by all three viruses. Compared with Yunnan and Guizhou provinces, apple samples collected from Sichuan province are most seriously infected by the apple latent viruses. The status of latent viruses infection in the main apple producing areas in Southwest China is first reported in this study, a set of rapid and effective molecular method for latent viruses identification is established.
【关闭】
