蝉花提取物通过促进氧化胁迫应答抑制H2O2诱导的HeLa细胞衰老
Cordyceps cicadae extracts has a protect effect against H2O2-induced cellular senescence by promoting oxidative stress response in HeLa cells
作者：王为岩(四川大学生命科学学院生物资源与生物环境重点实验室)；高雪洁(四川大学生命科学学院生物资源与生物环境重点实验室)；刘冰花(四川大学生命科学学院生物资源与生物环境重点实验室；成都大学医学院生物化学与分子生物学教研室)； 杨力(四川大学生命科学学院生物资源与生物环境重点实验室)； 闫孟力(四川大学生命科学学院生物资源与生物环境重点实验室)；刘科(四川大学生命科学学院生物资源与生物环境重点实验室)
Author：WANG Wei-Yan(Key Laboratory of BioResources and EcoEnvironment of Ministry of Education, College of Life Sciences,Sichuan University)；GAO Xue-Jie(Key Laboratory of BioResources and EcoEnvironment of Ministry of Education, College of Life Sciences,Sichuan University)；LIU Bing-Hua(Key Laboratory of BioResources and EcoEnvironment of Ministry of Education, College of Life Sciences,Sichuan University；2.Department of Biochemisty and Nolecular Biology,Medical College)；YANG Li(Key Laboratory of BioResources and EcoEnvironment of Ministry of Education, College of Life Sciences,Sichuan University)；YAN Meng-Li(Key Laboratory of BioResources and EcoEnvironment of Ministry of Education, College of Life Sciences,Sichuan University)；LIU Ke(Key Laboratory of BioResources and EcoEnvironment of Ministry of Education, College of Life Sciences,Sichuan University)
收稿日期：2017-04-05 年卷（期）页码：2018,55(3):632-636
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：蝉花；活性氧；抗氧化；抗衰老
Key words：Cordyceps cicadae; ROS; Antioxidant; Anti-aging
基金项目：成都市科技人才发展工程项目2015-RC02-00040-HZ
中文摘要
本实验探究蝉花提取物(CCE)通过促进HeLa细胞的氧化胁迫应答抑制H2O2诱导的细胞衰老.在实验中，使用不同浓度的CCE处理HeLa细胞48h或72h，检测细胞的存活率.然后使用H2O2在HeLa细胞中诱导氧化胁迫，检测CCE处理组和对照组细胞的β 半乳糖苷酶活性和ROS水平的变化.HeLa细胞经CCE处理72h之后，提取RNA，通过实时荧光定量实验检测CAT、SOD1、SOD2、GPX1等抗氧化基因的表达量.结果表明：CCE抑制HeLa细胞的增殖，且呈剂量依赖效应，较低浓度的CCE（≦0.100mg/mL）对细胞生长无明显抑制作用.0.100mg/mL的CCE处理HeLa细胞72h后能够显著地促进CAT和SOD1 等基因的转录，从而降低H2O2产生的过量ROS，抑制细胞衰老.
英文摘要
This study aims to investigate the protective role of Cordyceps cicadae extracts(CCE)against H2O2 induced oxidative stress in HeLa cells.HeLa cells were treated with CCE for 48h and 72 h to check cell viability.The SA β GaL activity and intracellular ROS was detected under the microscope after H2O2 treatment.The mRNA levels of CAT、SOD1、SOD2 and GPX1 were measured by real time PCR in HeLa cells after CCE treatment.Results show that CCE inhibited the growth of HeLa cells in a dose dependent manner.Pre treated HeLa cells with CCE (0.100mg/mL) showed decrease cellular senescence and level of ROS against H2O2 induced oxidative stress.CCE can increase CAT and SOD1.Our results indicate that the CCE has protective effect against H2O2 induced cellular senescence in HeLa cells.
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