二甲双胍与白花丹参联合治疗2型糖尿病的研究
Combined utilization of metformin and Salvia miltiorrhiza bge. f. alba on diabetes mellitus
作者：麻建平(浙江大学医学院附属二院建德分院;浙江省建德市第一人民医院)
Author：MA Jian-Ping(The Second Affiliated Hospital Jiande Branch, School of Medicine, Zhejiang University; The First People’s Hospital of Jiande)
收稿日期：2017-08-27 年卷（期）页码：2018,55(5):1116-1120
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：二甲双胍； 白花丹参； 糖尿病； 胰岛B细胞； 抑制凋亡
Key words：Metformin; Salvia miltiorrhiza bge. f. alba; Diabetes; Islet B cells; Inhibition of apoptosis
基金项目：浙江省医学会临床医学基金（2015ZYC-A45）
中文摘要
为了探究二甲双胍与白花丹参浸出液联用对2型糖尿病的治疗作用, 本文采用MTT法检测联合用药对INS 1细胞凋亡的抑制作用, 并采用糖尿病大鼠模型研究联合用药对血糖和胰岛素释放的影响. 结果表明, 二甲双胍与白花丹参联用具有抗INS-1细胞凋亡的作用. 在糖尿病大鼠治疗过程中, 检测血糖和胰岛素含量可知二甲双胍与白花丹参联用能有效控制血糖, 效果比单独使用二甲双胍明显. HE染色也显示联合用药能抑制胰岛细胞凋亡, 且比二甲双胍或白花丹参单独使用的效果好. 免疫组化染色中, 与糖尿病对照组和二甲双胍组相比, 联合用药组的Bcl 2蛋白量增加, Bax蛋白量减少. 二甲双胍与白花丹参联用具有抑制胰岛B细胞凋亡, 增加胰岛素释放的作用, 同时促进血糖摄取和利用, 能起到很好的降血糖作用. 白花丹参作为治疗糖尿病的辅助药物能有效提高二甲双胍的治疗效率.
英文摘要
In order to observe the combined utilization effect of metformin and Salvia miltiorrhiza bge. f. alba on diabetes mellitus type 2, MTT method, glycemic and insulintest, hematoxylin eosin (HE) staining and immunohistochemical staining were carried out in this paper. MTT test results show that combined utilization of metformin and Salvia miltiorrhiza bge. f. albacan inhibit the apoptosis of INS-1 cells. In the treatment of diabetic rats, combined utilization appears to effectively control to blood sugar. HE staining and immunohistochemical staining results show that Salvia miltiorrhiza bge.f. alba can promote the production of Bcl-2 proteins, weaken the ability of Bax proteins to induce apoptosis, in the mean time, metformin has the effect on increasing blood sugar uptake and utilization, ultimately achieve glycemic control purposes. Combined utilization of metformin and Salvia miltiorrhiza bge. f. alba has the potential to be an effective method to the treatment of diabetes.
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