大孔树脂纯化天麻多糖的工艺研究
Macroporous adsorption resin for the purification of polysaccharides from Gastrodia elata Bl
作者：陈琛(陕西理工大学生物科学与工程学院/陕西省天麻山茱萸工程技术研究中心/陕西理工大学中德天然产物研究所)；李鑫鑫(陕西理工大学生物科学与工程学院/陕西省天麻山茱萸工程技术研究中心/陕西理工大学中德天然产物研究所)；魏唯(陕西理工大学生物科学与工程学院/陕西省天麻山茱萸工程技术研究中心/陕西理工大学中德天然产物研究所)；刘祥(陕西理工大学生物科学与工程学院/陕西省天麻山茱萸工程技术研究中心/陕西理工大学中德天然产物研究所)；田慧玲(陕西汉王药业有限公司)；冯振斌(陕西汉王药业有限公司)；胡红忠(陕西百圣生物工程有限公司)；郑红星(陕西理工大学生物科学与工程学院/陕西省天麻山茱萸工程技术研究中心/陕西理工大学中德天然产物研究所)
Author：CHEN Chen(Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology)；LI Xin-Xin(Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology)；WEI Wei(Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology)；LIU Xiang(Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology)；TIAN Hui-Ling(Shaanxi Hanwang Chinese Medicine Co.,Ltd)；FENG Zhen-Bin(Shaanxi Hanwang Chinese Medicine Co.,Ltd)；HU Hong-Zhong(Shaanxi Baisheng Biological Engineering Co.,Ltd)；ZHENG Hong-Xing(Chinese German Joint Institute for Natural Product Research/Shaanxi Engineering Research Center of Tall Gastrodia Tuber and Medical Dogwood/College of Biological Science and Engineering, Shaanxi University of Technology)
收稿日期：2017-10-06 年卷（期）页码：2018,55(5):1109-1115
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：多糖；大孔吸附树脂；吸附；纯化；天麻
Key words：Polysaccharide; Macroporous resin; Adsorption; Purification; Gastrodia elata
基金项目：国家高端外国专家项目（GDW20146100228，GDT20176100048）；国家重点引智项目（W20166100045）；陕西省科技统筹创新工程计划项目（2015KTTSSF01-03，2015HBGC-18）；陕西省重点研发计划项目（2016NY-180）；陕西省协同创新计划项目（2016XT-13）；陕南秦巴山区生物资源综合开发协同创新中心资助项目（QBXT-Z(P)-15-21）
中文摘要
本研究以炮制的干天麻为原料，水提醇沉法提取多糖，大孔吸附树脂纯化，比较了八种大孔树脂（AB-8、D101、LX-17、D301、NKA-9、S-8、LSD-001、ADS-7）对天麻多糖静态吸附 解析效果，筛选出最佳纯化树脂，再研究最佳树脂纯化天麻多糖工艺参数.结果为：八种大孔吸附树脂中D101对天麻多糖的纯化效果最好.样品液浓度、温度、上样速度，洗脱用乙醇浓度、洗脱流速及洗脱体积等因素均对D101树脂吸附分离天麻多糖有影响.所得的最佳纯化工艺为：20 ℃是较适宜的吸附温度，上样速度1 BV/h，上样浓度4 mg/mL，进行吸附；吸附饱和平衡后，用解析液浓度60%乙醇，解析速率2 BV/h，解析液体积3 BV进行动态洗脱.通过该工艺天麻多糖的纯度提高到了65.7%，表明了大孔树脂D101对天麻多糖具有较好的纯化效果.
英文摘要
The Gastrodia elata polysaccharides were purified using a macroporous adsorption resins method. By comparing the Gastrodia elata polysaccharides separation effect of eight different types （AB-8、D101、LX-17、D301、NKA-9、S-8、LSD-001、ADS-7）of macroporous resin, D101 was selected as the ideal adsorbent as it had a strong adsorption ability and a high desorption rate. The optimal purification technology was as follows: loading rate1 BV/h, loading concentration 4 mg/mL, 60% ethanol as suitable elution solvent from the resin, the elution solvent volume 3 BV and the elution flow rate 2 BV/h. The purity of Gastrodia elata polysaccharide was increased from 15.6% to 65.7%, which indicated that macroporous resin D101 had better purification effect on Gastrodia elata polysaccharide.
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